Armored Technology™
Overview
Armored Technology provides:
- Protection of RNA targets from degradation – enabling stable RNA-based assay controls
and standards
- Customizable RNA sequences – design controls specifically around your assay needs
- Non-infectious configuration – enabling safe handling and easy shipping
Armored RNA® is based on bacteriophage coat protein encapsulation of specific RNA
targets to form pseudo-viral particles. The bacteriophage coat protein protects
RNA transcripts from nuclease degradation and can stabilize RNA sequences.
Armored RNA® is designed for use as standards and controls in assays, in particular
for use as positive controls or quantitative internal spiked controls for amplification
and detection using RT-PCR.
References:
WalkerPeach CR, Winkler MM, DuBois DB, Pasloske BL. Ribonuclease resistant RNA controls
(Armored RNA) for RT-PCR, bDNA and genotyping assays for Hepatitis C Virus. Clin.
Chem. 45:2079-2085, 1999.
Pasloske BL, WalkerPeach CR, Obermoeller RD, Winkler M, DuBois DB. Armored RNA technology
for the production of ribonuclease resistant viral RNA controls and standards. J.
Clin. Microbiol. 36:3590-3594, 1998.
Two versions of Armored RNA® are available to meet your specific needs:
Armored RNA® Quant™ Applications:
- Quantitate samples against standard curve
- Internal Control to confirm negative results
- External Extraction Control to determine extraction efficiency
- External Control for direct detection of target
Armored RNA® Quant™ provides:
- Package target RNA of choice or ready-to-use products for popular targets
- Greater than 2000 bp insert target size
- Manufactured according to QSR guidelines
- Qualification using National Institute of Standards and Technology (NIST) traceable
reference standard
- Custom fill and finish service available
Armored RNA® Quant™ technology stabilizes RNA transcripts from nuclease degradation
by packaging them in a protective protein coat. The packaging of the RNA transcripts
is performed in-vitro, enabling very tight control of RNA quality packaging. Due
to the added stringency of this preparation, Armored RNAsup>® Quant™ can be directly
quantified via a primary phosphate standard certified by the National Institute
of Standards and Technology (NIST).
Armored RNA® provides:
- Package target RNA of choice or ready-to-use catalog products for many applications
- 150 to 500 plus bp insert target size
Armored RNA® technology stabilizes RNA transcripts from nuclease degradation by
packaging them in a protective protein coat. The packaging of a cloned RNA target
sequence is driven off an inducible expression plasmid in host E coli.
