miRInform™ Data Delivery System

miRInform™ was designed to help clients understand and manipulate the data generated by miRNA Expression Profiling. The results of your study are organized and delivered in an easy-to-access format.

Explore your data by selecting straight from interactive data graphs and tables to review miRNA database entries on the most differentially expressed miRNA, sortable tables, and online help files.

Description of Services

  • Features of the miRInform System:
    • Understand your data within minutes
    • Fast, cost-effective analysis for a wide range of experimental designs
    • Normalization optimized for miRNA analysis
    • Interactive figures linked directly to Sanger miRBase
    • Publication-quality images included
    • Know your signal processing and summarization were performed appropriately
    • Available for the DiscovArray and Agilent miRNA profiling services
  • Workflow for the miRInform System:
    • Data summarization (if necessary)
    • Normalization by VSN
    • Calculation of QC metrics
    • Statistical analysis by one-way or multi-way ANOVA
    • Generation of reports and graphs

Deliverables

  • Raw array data files (see miRNA Services)
  • Bioanalyzer PDF files (for projects including RNA isolation services from non-FFPE samples)
  • Analysis Report Tables (in HTML format and MS Excel format, unless table dimensions exceed MS Excel capabilities, in which case tab delimited files will be provided)
    • Experimental design
    • Array QC Summary
    • Control Signals
    • Raw Signal and Detection calls
    • Statistics (not included if experimental design does not allow statistical analysis, or in data-only projects)
    • Pearson Correlation coefficients
    • Probes Detected by Source
    • Normalized Array Data
    • Comprehensive Annotation to the most recent release of Sanger miRBase
  • Figures
    • Static figures (in .jpg, .eps and .png format):
    • Data enhanced volcano plot(s)
    • Data enhanced MA plot(s)
    • Hierarchical Clustering, all detected probes
    • Pearson Correlation coefficients
    • Principal Components Analysis (PCA) Plot(s): A) All detected probes and B) All probes passing significance testing if experimental design allows statistical analysis
    • Box Plots: Before and after normalization
  • Interactive figures (Java™ enabled) - High quality figures are ready for publication directly onto your desktop or PowerPoint. Spend your valuable time reviewing the results rather than generating figures from your data.
    • Volcano plot(s) with hover-over probe identification and hyperlinks to Sanger miRBase
    • MA plot(s) with hover-over probe identification and hyperlinks to Sanger miRBase
    • Hierarchical Clustering plots with interactive drill-down enhanced visualization
    • PCA Plots with hover-over sample identification

Example Volcano Plot from miRInform:

miRInform contains many data-rich volcano plots, which show p-value and fold change between two groups for all miRNAs. Color and spot diameter add information about mean expression level and percent detected, respectively in static plots. Validated Sanger content is hot linked to the Sanger miRBase.

Threshold and Normalization

For experiments involving hypothesis testing, miRInform offers unprecedented value. miRInform captures the results from Asuragen’s statistical analysis for a wide range of study designs.

  • Recommended thresholds for p-value and fold-change are provided, but these can be adjusted for your unique study needs
  • Recommended normalization method is global in nature to maximize precision within a study, but array normalization also can be specified
  • Array-based normalization instead if it suits your downstream analysis
  • Optimized for experiments with fewer than 100 samples and 10 experimental groups
  • Our informatics staff can easily accommodate any study design and size

Confused about these choices? Contact us for a free consultation to determine what is right for you.

Quality Control

Rigorous procedures enable Asuragen to be the only service provider to offer a Data Quality Guarantee:

If, for any reason, a qualified sample does not pass our process control specifications, we will re-run the sample at no additional charge.

Read more about our commitment to the best possible data quality, or read about our contributions to in-process controls and standardization.

Turn-around time

Most projects are completed in one week or less. If you have a critical time requirement, we can often meet your need for no additional charge. Read more about our commitment to prompt turn-around times.

Additional Services Available

Catalog Numbers for miRNA Informatics Services

Pathway Analysis:

CS0004 Data Analysis, microRNA, Standard Service Premium
CS0020 Data Analysis, miRInform™ package