Sample Preparation and Isolation of RNA or DNA
Eukaryotic and Prokaryotic Cell Lines
Cell lines offer an accessible, easy-to-use set of models for biological study and
can provide sufficient quantities of RNA/DNA for almost any application. Asuragen
Services staff has performed RNA/DNA isolation from cultured cells for over 15 years.
Learn more about how
we qualify our methods and staff.
Description of Services
Features of these sample prep services:
- RNA isolation methods optimized to recover miRNA
- Asuragen-isolated RNA or DNA suitable for numerous downstream
applications, including expression profiling and genotyping studies (additional
services)
Workflow for sample prep:
- Cell disruption (if required)
- Column-based isolation and cleanup
- DNAse treatment (if applicable)
- QC by NanoDrop spectrophotometer and Agilent 2100 Bioanalyzer
- Re-purification and/or concentration if required
Deliverables
Regardless of downstream application, detailed QC reports including nucleic acid
concentration, RNA integrity (RIN), electropherograms (where applicable), and purity are
provided on each sample. Purified nucleic acid samples are shipped back to you on
dry ice. Asuragen ships samples Monday through Wednesday and will notify you upon
shipment.
Isolation services are often utilized with Asuragen’s
gene expression services,
miRNA profiling services, or
genotyping services. Surplus RNA or DNA will be discarded unless
otherwise requested. Additional shipping charges may apply for the return of residual
blocks, RNA, or DNA.
Quality Control
No matter how robust the downstream processes, good data is dependent on sample
quality; consequently, accurate measurement of sample quality is essential before
deciding if a sample merits further analysis.
For RNA
All samples prepared by Asuragen Services are routinely qualified by spectrophotometry
with the NanoDrop spectrophotometer
and the Agilent 2100 Bioanalyzer. If you have chosen to use Asuragen
Services for additional services and a sample does not pass the recommended QC metrics
for analysis, you will be contacted promptly to discuss possible solutions. These
may include replacing the sample or re-isolating the RNA, excluding the sample from
further analysis, or proceeding at-risk.
High Quality RNA
RNA was isolated from colon tumor tissue by Asuragen and run on the Agilent 2100
BioAnalyzer to show integrity (red line). The same RNA was incubated overnight at
37°C to show stability (blue line). This data shows that there was no change in
RNA quality and integrity after extended incubation.
For DNA
All DNA samples prepared by Asuragen Services are routinely qualified by spectrophotometry
with the NanoDrop spectrophotometer
and gel electrophoresis. Due to the degraded nature of DNA from FFPE tissues, special
quality control measurements are used.
These rigorous procedures enable Asuragen to be the only service provider to offer
a Data Quality Guarantee:
If, for any reason, a qualified sample does not pass our process control specifications,
we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
standardization.
Turn-around Time
Most projects are completed in one to two weeks. If you have a critical time requirement,
we can often meet your need for no additional charge.
Read more about our commitment to prompt turn-around times
Typical Yields
Human Cell Lines Standard Yields*
* Yield information derived from large scale preps; these values should be used
as a nominal guide. Please consult with our technical experts prior to submitting
samples.
Additional Services Available
mRNA Analysis:
Affymetrix Gene Expression Profiling Service
Illumina Gene Expression Profiling Services
Applied
Biosystems TaqMan Gene Expression Services
miRNA Analysis:
Ambion/Affymetrix
DiscovArray miRNA Expression Profiling
Agilent miRNA Microarray Service
Applied
Biosystems TaqMan miRNA Expression Services
DNA Analysis:
Agilent aCGH
Affymetrix SNP 6.0 - coming soon
Data Analysis:
Asuragen’s Informatics
Team offers a broad range of analytical services to help you get the most information
from your data.
Catalog Numbers for Cell Line Sample Prep Services
|
CA0041
|
Isolation, Total RNA from cell pellet
|
|
CA0025
|
Isolation, DNA
|
Requirements for Cell Line Sample Prep and Related Services
Sample Input Requirements for the services listed above are found in the Sample Submission Form and Shipping Instructions.